Ubiquitin AMC 81150 from BPS Bioscience, Inc.

Description

Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). Upon incubation with a protease recognizing Ubiquitin, such as USP2 or UCHL3, AMC is released and the increase in fluorescence can be measured using Ex380 nm and Em460 nm wavelengths. This protein contains no extraneous tags

Product Specs
ItemUbiquitin AMC
CompanyBPS Bioscience, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Number81150
Quantity50 µg
ActivityTypical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM
Purity≥95% by HPLC
Molecular Weight8722 Da
SpeciesHuman
TypeProtein
ApplicationsUseful as a substrate for deubiquitinating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs), as well as investigation of deconjugating enzyme substrate specificity and for screening for modulators of
NCBI Full Gene Nameubiquitin A-52 residue ribosomal protein fusion product 1, ubiquitin C-terminal hydrolase L1
NCBI Gene AliasesPGP95, L40, PGP9.5, PARK5, CEP52, HEL-117, HEL-S-53, Uch-L1, PGP 9.5, SPG79, NDGOA, HUBCEP52, RPL40